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All protein spots identified as significantly different (FC 0.30 and P 0.05) were selected for MS analysis

(3) 72 hours later, the rats are anesthetized and immediately perfused transcardially with 50 mL 0.9% saline followed by 500 mL 0.1 m neutral phosphate-buffered formaldehyde (4%)

The work focuses on mechanisms by which DNA methylation, histone methylation, and noncoding RNAs converge in a number of overlapping and self-reinforcing pathways to promote stable and heritible gene silencing

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Results were gradual but very visible

Proteomic analysis of tears showed suppression of the complement cascade, and of neutrophil and platelet activation mediators [73]
