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The blocking solution was discarded, and the secondary primary antibody was added

doi: 10.1016/j.addr.2008.06.002 155 Comas-BastOSnchez-PrezSVeciana-NogusMTLatorre-MoratallaMVidal-CarouMDC

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[DOI] [PubMed] [Google Scholar] Schroeder JP, Overstreet DH, Hodge CW (2005) The neuropeptide-Y Y5 receptor antagonist L-152,804 decreases alcohol self-administration in inbred alcohol-preferring (iP) rats

The unbound GCs are small and lipid-soluble, and can easily diffuse through lipid-rich cell membranes via passive intracellular diffusion