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Proteins were denatured in sample buffer and equal amounts were separated on SDS-PAGE and transferred to PVDF membrane (BioRad Laboratories, CA, USA)

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Daily functioning should become noticeably easier as well once the vitamin levels are maintained at a healthy level

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However, many other phytochemicals more directly target inflammation: celastrol, for example, powerfully inhibits NFB, a master regulator of inflammation, thereby reducing downstream cytokines and slowing neurodegenerative inflammation [51]