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For instance, only patients with a BRAF V600E -mutated melanoma are expected to benefit from targeted therapies with BRAF/mitogen-activated protein kinase (MEK) inhibitors, while patients with a BRAF K601E -positive melanoma respond only to a minority of those drugs, such as trametinib ( V600E detection through circulating tumor DNA prior to treatment is predictive of response to BRAF/MEK inhibitors (218)

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Dose-response assays were conducted beforehand to establish the highest nontoxic and effective concentrations, which were then used in all experiments