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PLoS ONE 8 , e79167e79167 (2013)

Theres no complicated routine to remember

Calculating the GSH/GSSG ratio from these measurements provides a reliable indicator of intracellular redox status in preclinical models

Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40

Furthermore, the regulatory status of these peptides is complex
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