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Oral corticosteroids (prednisone) provide short-term anti-inflammatory benefit but have significant side effect profiles with repeated courses, including bone density loss, immune suppression, and metabolic disruption

by UVVis spectrophotometry with comparative study on different extraction technologies

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Following treatment, the cells were washed with PBS, fixed with 4% paraformaldehyde and stained with 0.3% ORO for 15 min to visualize lipid accumulation

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They are zinc-dependent exopeptidases that exhibit distinct substrate specificities: CCP1, 2, 3, 4, and 6 preferentially shorten -linked glutamate side chains, while CCP5 uniquely removes the branching -linked glutamate that initiates chain formation [24, 25, 33, 34] (Fig