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Although one of these sites is located in the 5 promoter region and the other site in the 3 flanking region, both sites are functional, as they have both been shown to bind the PU.1 nuclear factor in gel mobility shift assays, and both mediate transcription, as assessed by reporter gene assays

Eisenach et al., 2017

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Tirzepatide begins working on a similar timeline to semaglutide, with some patients reporting appetite changes almost immediately and significant weight changes becoming visible by weeks six through twelve