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The activity of CAT takes place in two steps

After infection with SARS-CoV-2, numerous cells, including epithelial cells, macrophages, T lymphocytes, neutrophils, and Th17 cells, release these proinflammatory cytokines in an unregulated manner, which worsens SARS-CoV-2 infection [97]

Importantly, supplementation can **increase** measurable NAD+ metabolism: in a 2018 randomized, placebo-controlled crossover trial published in Nature Communications by Martens and colleagues at the University of Colorado Boulder (24 healthy middle-aged and older adults), 1,000 mg/day of the NAD+ precursor nicotinamide riboside (500 mg, twice daily) elevated NAD+ in blood cells by roughly 60% versus placebo over 6 weeks, with no serious adverse events reported

, shifts in microbial taxa and functional dysbiosis i.e

Membranes were incubated with primary antibodies overnight at 4 C, followed by incubation with secondary antibodies conjugated with either horseradish peroxidase or fluorescent dye
Our observations are further supported by a study in APPswe/PS1dE9 mice [78]