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For targeted metabolomic analysis of 40 acylcarnitines, 42 amino acids and biogenic amines, 90 glycerophospholipids, 15 sphingolipids, and 1 monosaccharide, samples were prepared using the AbsoluteIDQ p180 kit (Biocrates Life Sciences AG) according to the manufacturers instructions and analyzed using flow injection analysis (FIA)-tandem mass spectrometry (MS/MS) and HPLC-MS/MS (AB Sciex 4000 QTrap mass spectrometer, Sciex, Framingham, MA, USA)

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198 LaurikainenH.TuominenL.TikkaM.MerisaariH.ArmioR

Consequently, 10 children completed the full three-month GF-MKD intervention , forming the final intervention cohort included in the present plasma metabolomic analysis

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However, NAC has low bioavailability in contrast to its amide derivative [19]