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When the NLRP3 inflammasome is activated, it activates caspase-1, which then forms active forms of pro-inflammatory cytokines such as IL-1 and IL-18 [186]

[PubMed] [Google Scholar] 9.Froesch ER, Burgi H, Ramseier EB, et al

The result is a drastically blunted, almost negated, GH pulse

The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

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A linear gradient of 0.1% TFA in water (Solvent A) and 0.1% TFA in acetonitrile (ACN) (Solvent B) was used, starting at 99% A with 1% B and increasing per minute at a flow rate of 1 mL/min and detection at 215 nm