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Acquired mutations in TET2 are common in myelodysplastic syndromes

Briefly, FreSHtracer was synthesized by mixing a coumarin-3-carbaldehyde derivative and 2-cyano- N,N -dimethylacetamide at 60 C for 2 days, and purified using SiO 2 chromatography [19]

Weiss M

Inability of myalgic encephalomyelitis/chronic fatigue syndrome patients to reproduce VO(2)peak indicates functional impairment

Cells were washed twice with the FLICA kit wash buffer and then incubated with LIVE/DEAD Fixable AQUA Dead Cells Stain (Thermo Fisher, USA) for 15 min at RT, followed by extracellular staining in PBS + 1% BSA with the following fluorochrome-conjugated antibodies for monocyte phenotyping: anti-CD14 BV605 [Clone: M5E2], anti-CD16 PE-Cy7/BV711 [Clone: 3G8], anti-CD3 PE [Clone: HIT3a] and anti-CCR2 PerCP Cy5.5 [Clone: K036C2] are from BioLegend
