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Combined with the role of IGF/IGF-1R in DRG and spinal cord, IGF/IGF-1R in different regions of the brain may play different roles in regulating pain, which also needs further research in the future
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One ml aliquot of the supernatant was neutralized with 1.5 ml of 0.5 M phosphate buffers (pH 7.5), then 50 l H 2 O was added
