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The hemibrain pellets were immediately lysed in 100 L of 1% SDS buffer (1% SDS in IP Lysis Buffer supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 100 mM N-Ethylmalamide), and homogenized with a pestle then boiled at 95 C for 10 min

Mol Biol Rep 49:78377848

30 mW cm 2 , 10 min)

Remarkably, this rate has seen little improvement over the past three decades [1, 2]

During the tendon repair, the experimental groups were injected around the Achilles tendon with 350mmol/L (low dose group) and 1000mmol/L (high dose group) D-ribose 0.2 ml respectively to increase the AGEs level, while in the control group were given the same amount of PBS