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doi: 10.1038/s41467-024-50098-2

After washing with PBS for 3 times, the tissue sections were incubated with the corresponding secondary antibodies at 37 C for 1 h

Zhang, H., Delafield, D

Amino Acids 42:181197 Szoke K, Hartel K, Grass D, Hirrlinger PG, Hirrlinger J, Hulsmann S (2006) Glycine transporter 1 expression in the ventral respiratory group is restricted to protoplasmic astrocytes

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The catalytic role of the enzyme provides high sensitivity and the possibility of varying detecting time to improve assay sensitivity