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The PCR protocol consisted of: a pre-heating step at 95C for 1.5 min, 35 cycles of heating at 95C for 30 seconds, cooling at 64C for 30 seconds, elongation at 72C at a rate of 1 kb/min, and a final elongation step of 5 minutes at 72C

Pre-weighed hearts and specimens of cortices as well as midbrains (or pelleted cells) were homogenized on ice in the 1 reaction buffer provided, using a Dounce homogenizer (3 times volume to weight ratio)

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Consistently, we found that knockdown of these enzymes rescued melanoma cells cultured under high glutamine, while having no effect on cells cultured in low glutamine (Fig

Glutathione is the liver's major antioxidant, often depleted in fatty liver disease due to oxidative stress and inflammation