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And the cut-off value of 0.13 was used as a standard for the subsequent tests (Figure 2B)

The precipitate was washed once with absolute ethanol and twice with ultrapure water, resuspended in an appropriate amount of ultrapure water, and stored at 4 C

(13) reported a significant reduction in the inflammatory response following the administration of a low dose of E2 to ovariectomized rats, indicating a more reliable outcome and suggesting that estrogen may mitigate hypoxia-induced inflammatory responses in the lung

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Orio et al., 2009)

This reaction results in the amino nitrogen remaining with the -carbon of 2-oxoglutarate, producing glutamate and -aminoadipic-6-semialdehyde