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Circulating T cells exhibit elevated expression of activation markers, such as CD69 and CD38, but there is a notable reduction in interferon- production upon stimulation [95, 96]

Cells were lysed by the addition of 200 L pre-cooling NADH/NAD + lysis buffer and centrifugation at 12,000 g for 10 min at 4 C

For immunotherapy, while the response can be durable, only a small fraction of the treated patients respond, and the approach works for only a few types of cancer (Rizvi et al

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