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Generation of deletion or GFP knock-in alleles by CRISPR/Cas9-mediated genome editing To make deletion alleles of sms-1 , sms-3 , and sms-5 , we generated P eft-3 - cas9 -NLS-pU6-sgRNA vectors carrying the sgRNAs targeting each gene by site-directed mutagenesis of pDD162 (#47549

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Relative to Day 1 fed animals, the average volume and number of DHS-3::GFP positive lipid droplets after a 24-h fast were reduced by 84% and 65%, respectively, as determined by fluorescence microscopy (Fig

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