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The day before conducting the main experiment, mice underwent three consecutive trials (with a 1-min intertrial interval) of 2 min at a speed of 12 rpm to habituate to the lowest functioning speed on the apparatus

doi: 10.1016/j.psj.2024.104521 78

To directly assess whether l-carnitine could induce a histopathologically ameliorative effect on synovitis, HE, Masson, and Sirius Red staining were applied

This modulation leads to the production of short-chain fatty acids, such as butyrate, acetate, and propionate, which are crucial for maintaining intestinal cells, adhesion molecules, and immunoregulation, particularly given the proximity of the gut-associated lymphoid tissue (205, 207)

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