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Folates play an important role in DNA synthesis (through pyrimidine synthesis), protection from DNA damage (though DNA-stabilizing polyamines), and DNA methylation (also through polyamine methyl donors), and upregulated folate metabolism may be indicative of the cells need to repair DNA damage

The SFN level was obtained by high-performance liquid chromatography and the injection volume was 10 L

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Supplementation of spermidine at 40 mg/day has minimal effects on circulating polyamines: an exploratory double-blind randomized controlled trial in older men

Toxicol Appl Pharmacol 296:5460 Wise SS, Aboueissa AE, Martino J, Wise JP Sr (2018) Hexavalent chromium-induced chromosome instability drives permanent and heritable numerical and structural changes and a DNA repair-deficient phenotype