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Yes, split dosing is a valid approach, particularly for doses at the higher end of the range (450-500mcg daily)
1999 Leane Hoey, J J Strain, Helene McNulty

1 The British Society for Haematology does not recommend retesting vitamin B 12 levels after treatment has been initiated, and no guidelines address the optimal interval for screening high-risk patients

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The cell and animal data line up reasonably well
