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After centrifugation at 1,000 g for 30 min at room temperature, the neutrophil-enriched cell pellet, appearing as a cloudy layer at the bottom of the tube, was collected and washed with HBSS

Research protocols for DSIP typically range from 25g to 500g per injection, depending on the endpoint being measured (sleep architecture, cortisol modulation, stress response)

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Chen, Z., Brodie, M

Such cells essentially become addicted to MYC amplification

This mechanism underscores their definite origin in contrast to other types of EVs