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Cell viability assays Eighty microliters of cell suspension (4 10 5 cells/ml) were seeded in a 96-well cell culture plate, followed by the addition of 20 l of drug solution in culture medium

A significant obstacle to achieving this is the susceptibility of probiotics to a confluence of hostile conditions, including gastric acidity, bile salts, temperature variations, and antimicrobial factors such as hydrogen peroxide, which can severely diminish their viability and functionality (273)

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Functionalization of nanoparticles with targeting ligands, such as antibodies or peptides, allows for site-specific accumulation and cellular internalization of the peptide cargo

The rate of dissociation of the monomeric avidin-biotin complex is sufficiently fast to effectively allow recovery of all bound protein in neutral pH and low salt conditions