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For bacterial expression, human DIDO3 SPOC (10471205 aa), SHARP SPOC (34963664 aa), RBM15 SPOC (775960 aa), and SPOCD1 SPOC (8581025 aa) were cloned into pET M11 between NcoI and XhoI for N-terminal His6 fusion

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Despite compelling mechanistic foundations for MNP effects, confirmation of a causal link in human populations requires large, standardized epidemiological and experimental studies