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Once it is inside the pore, salicylic acid does three things at once

The catalytic cysteine is highly susceptible to oxidation and nitrosylation, leading to reversible or irreversible modifications that abolish its nucleophilic function and inactivate its enzyme activity (87, 88)

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The tissue becomes lumpy and firm

To calculate the DPG, proximal skin temperature was subtracted from the distal skin temperature
