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Sandoval and Klaus Weber reported in 1978 that large microtubule-associated proteins MAP1 and MAP2 (that are crucial for tubulin assembly) are rapidly degraded in crude brain extracts by a calcium-dependent protease, which does not affect tubulin, and this event results in the permanent loss of tubulin polymerization (Sandoval and Weber, 1978)

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If the resulting injection volume is too large, you may want to start with a fresh vial and use less water

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