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Then, two wells from each condition were thoroughly resuspended by pipetting and OD 600 readings for those wells were taken on a Biotek Synergy 2 plate reader
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GHK-Cu does not 'activate a receptor' that turns off when the ligand dissociates

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High resolution imaging of subcellular glutathione concentrations by quantitative immunoelectron microscopy in different leaf areas of Arabidopsis
