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doi: 10.1016/S0092-8674(01)00200-8

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Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)

The genome of Vibrio is organized into a two-chromosome configuration