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The ability of benfotiamine to inhibit three major pathways (the hexosamine pathway, the AGE formation pathway, and the diacylglycerol (DAG)-PKC pathway) simultaneously might be clinically useful in preventing the development and progression of diabetic complications
Once the binding interaction finish, the additional buffers are utilized to wash support and remove non-bound components in the samples
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Shin JM, Cho YM, Sachs G (2004) Chemistry of covalent inhibition of the gastric (H+, K+)-ATPase by proton pump inhibitors
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