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TLR2 pep-Cys was chemically synthesized on solid phase

The flow-through was discarded, and then the filter unit was incubated with 40 L of 50 mM AmBic with trypsin (1/100 concentration of protein) overnight at 37 C

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He then became a postgraduate student at the Institute of Microbiology, where he met his future wife, Hana, and started a family (Lefkovits 2025)

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