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Equal amounts of cell lysates were prepared using a protein loading buffer, and the samples were homogenized to a final concentration of 2 g/L

Isobolographic analysis was implemented to determine if the interaction between two drugs given in combination is synergistic (supra-additive), additive, or antagonistic (infra-additive) [26, 27]

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All these procedures are best done under live image-guidance to ensure accurate and specific targeting of the injury site with appropriate localization of cells and scaffolding with growth factors for optimal regeneration and healing

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