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doi:10.3390/pharmaceutics15020533 133

After three washes with PBT, the sections were incubated with the Alexa Fluor 546-conjugated anti-rabbit (1:600) and Alexa Fluor 488-conjugated anti-mouse (1:400) secondary antibodies for 2 h at room temperature in the dark

Common ingredients in these products (caffeine, synephrine, yohimbine) can increase heart rate and blood pressure

The high sensitivity (100% for the known carcinogenic and mutagenic NAs tested here) of HepG2 cells supplemented with hamster S9-mix, as compared to PHH and PRH, in principle might make this system a valuable tool for hazard identification and potential follow up of negative/borderline EAT test results to provide further reassurance for the EAT result

Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research