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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen, and Kaleidoscope, BioRad)

GHK-Cu is a copper-binding peptide complex widely studied for its role in cellular signaling, protein interaction, and tissue-related pathways within controlled laboratory environments

The frequency of B12 injections can vary based on individual needs, current B12 levels, and specific health goals

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Anyone with known copper allergy should avoid GHK-Cu

Stability note: Avoid repeated freezethaw cycles to help preserve peptide integrity