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To extract amino acids from cultured myotubes, cells were washed once with cold PBS, and then quenched with 100 L ice-cold extraction solvent (80:20, methanol:water) containing isotopically labeled internal standards (MSK-CAA-1, Cambridge Isotope Laboratories, all at 1 M)

[DOI] [PMC free article] [PubMed] [Google Scholar] 86.Ishii I., Akahoshi N., Yamada H., Nakano S., Izumi T., Suematsu M

In conclusion, gaining a comprehensive understanding of bidirectional kidney-liver crosstalk is pivotal for advancing patient care and enhancing outcomes in the realm of renal and liver diseases

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Neurobion injections help to prevent or treat vitamin B deficiency