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The cells were transfected with 3 g of AKT-siRNA (RIBOBIO, China) in 240 L OPTI-MEM Reduced Serum Medium for 57 h using a previously reported procedure

Pochini, L., Oppedisano, F

Collected CMs were used to assess their ability to induce capillary-like structures of HUVE cells on matrigel

The ante-MMPBSA.py [72] module of AmberTools 14 was used to convert the original topology of the system, remove all the waters and ions, specify the Born radii as mbondi3 , and generate the corresponding topology file to be used in the MM/GBSA calculations