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Sample Preparation Animals were fasted overnight and for clinical chemistry blood samples from each rat were collected from the eyes by retro-orbital puncture using blood capillary tubes without heparin as per requirement under mild ether anesthesia blood samples were incubated at room temperature for 10 minutes and left to clot then centrifuged at 3000 r.p.m for 10 min and the serum were collected, serum was separated and kept in clean stopper plastic vial at 80C until the analysis of serum parameters

Thus, the results supported our hypotheses

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A 2022 randomized controlled trial found that vitamins B1, B6, B12, and B9 (and a combination of these vitamins) could be an effective treatment for migraine but emphasized the need for further study

Bu rn, sporcularn performanslarn en st seviyeye karmalarna yardmc olmak iin zel olarak formle edilmitir