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If the blue color doesnt return and your column is still white after elution, this means that you stripped the nickel ions off of the agarose beads, likely by using too much EDTA in your buffers

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Key differences include: localized repair peptide vs systemic growth factor splice variant origin vs engineered analog PEGylated stability vs receptor affinity enhancement These differences define their roles within growth and regeneration research

John Merriam email: [email protected] phone: (310) 825-2256 office: 4309 LSB Research Interests The recent demonstration that eye tissue is formed after ectopic expression of the eyeless gene suggests that how tissues differentiate is driven by a limited number of "master" genes

Biochemistry 40:20522059