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For transfection, 2.5 g DNA pe r well in a 6-well plate, 1 g DNA per well in a 12-well plate, 100 ng DNA per well in a 96-well plate were utilized

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doi: 10.1038/s41593-019-0423-2 187 SeidelKBouzrouMHeidemannNKrugerRScholsLden DunnenWFAet al

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The neurons of cortical cerebral region, cerebellar region, hippocampus, and hypothalamus were counted in 10 different high-powered fields (HPF, 400), and 3 to 5 serial sections of each sample were used to perform the counting as described in [84]