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2025 Jan 1:282:126966

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in terms of quantity, there is a threshold effect: a study of Indian middle school students (28) indicated that when daily protein intake was 1.2 g/kg body weight, intestinal tryptophan supply was stable, 5-HT (5-hydroxytryptamine) synthesis was sufficient, and depression scores decreased significantly (mean difference = 1.8 points, P 1 g/day) has a small effect on moderate depression, while low-dose supplements ( 0.5 g/day) provide no benefitthis dose-response relationship was not discussed in the original analysis (65, 66)

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Primary antibodies were obtained as follows: Anti- ER-alpha, Anti-PARP, Anti-Cleaved Caspase-3, Anti-PARP and Anti-P-SAPK/JNK were from Cell Signaling (Danvers, MA, USA), Anti-4HNE and Anti -actin was from Abcam (Toronto, ON, Canada), Anti-GAPDH and Anti--Tubulin was from (Sigma), Anti-Nrf2 (H-300) and Anti-NQO1 (A180) were from Santa Cruz (Santa Cruz, CA, USA), Anti-Calnexin was from Enzo Life Sciences (Farmingdale, NY, USA) and Anti-GPR30 was from Genscript (Piscataway, NJ, USA)