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[DOI] [PMC free article] [PubMed] [Google Scholar] [60].Zhou X, Wang HY, Wu B, Cheng CY, Xiao W, Wang ZZ, et al

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To assess the inhibition of ongoing ATP hydrolysis catalyzed by Ceg14 by AnkJ, a reaction system containing 1 g Ceg14, 1 g actin, 1 mM ATP, 1 mM DTT, 5 mM MgCl 2 , 20 mM Tris-HCl (pH 7.5), and 100 mM NaCl was incubated at 37 C for the indicated time and 2 g AnkJ or an equivalent volume of dialysis buffer was then added, and the incubation was continued at 37 C for 2 h

Luzzatto L, 1973

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