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In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)

Female reproductive capacity declines with age as a consequence of an age-related decrease in oocyte quality and quantity, which is accompanied by mitochondrial dysfunction associated with decreased oxidative phosphorylation and reduced ATP level [222,223,224]

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These studies showed that the levels of CoQ10 increase progressively, starting in the first trimester and continue to rise until delivery (Table 2)

10.1021/bi00476a021 133 Van VeldhovenP

Determine the concentration of the controls and samples