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The plasmid DNA was linearised with the restriction enzyme Spe1 (New England BioLabs, Inc) and transcribed into mRNA by T7 RNA polymerase with the AMBION mMESSAGE mMACHINE TM T7 transcription kit (Invitrogen TM , Thermo Fisher Scientific)

While most PTT devices require extended periods of therapy up to 8 h per day, the RestoreX device can be effective at 3090 min per day

Of these, three fish per tank were designated for blood collection and tissue sampling (including liver, kidney, and intestine)

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