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Brain-derived neurotrophic factor protects dopamine neurons against 6-hydroxydopamine and N-methyl-4-phenylpyridinium ion toxicity: Involvement of the glutathione system

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A total number of 2635 raw movie stacks made of 50 frames each were collected with SerialEM 87 from a single individual session with a GLACIOS 200 kV FEG Cryo-TEM (ThermoFisher Scientific) using a K2 Summit detector (Gatan Inc., USA) at 36000 magnification and pixel size of 1.145 /pixel without pre-exposure and using a defocus of 2.6 to 1.8 m and 50 e - / 2 total dose per stack (1 e - / 2 per frame)

Researchers planning to buy Wolverine peptide stack receive a lot file showing both peaks resolved separately, two target masses confirmed in the spectrum, and a single combined endotoxin result rather than averaged or summed metrics