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Continue avoiding active acids and retinoids until day 4

However, a key unresolved question is how the specificity, reversibility, and spatiotemporal dynamics of SSG are coordinated across different cellular compartments and pathological contexts

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After the collection was finished, the filter, together with the suspended particulate matter, was conditioned again in an environmental chamber for 24 h

The peptides were then eluted through a C-18 column (Thermo Fisher Scientific, 50 1 mm Hypersil Gold C-18) using a rapid gradient from 10 to 90% acetonitrile containing 0.15% formic acid and a flow rate of 0.04 ml/min, leading directly into a maXis-II ETD ESI-QqTOF mass spectrometer (Bruker Daltonics)

Arterioscler Thromb Vasc Biol 25:21002105 Onay-Besikci A, Wagg C, Lopaschuk TP, Keung W, Lopaschuk GD (2007) Alpha-lipoic acid increases cardiac glucose oxidation independent of AMP-activated protein kinase in isolated working rat hearts