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qPCR was then performed using Power SYBR Green PCR Master Mix (Applied 509 Biosystems) in StepOnePlusTM Real-Time PCR Systems using respective qPCR gene specific primers

Physella acuta plays a key role in the ecosystems as a food source for larger species and by contributing to nutrient cycling (McClain et al., 2012

10.1089/ars.2005.7.999 60 HurdT
is accurate and current

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