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Back in 2020 I completed continuing-education training specifically on peptides, and I found the science genuinely fascinating

2 Human securin prevents premature chromosome segregation through the inhibition of separase, a protease that cleaves cohesin, which links sister chromatids

Western blot Cells were lysed by RIPA lysis with 1% PMSF on ice for 30 min, and then centrifuged with 12,000 g at 4 C for 30 min
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Pushpakumar et al., 2017)