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As an experimental system we used a human BBB co-culture model improved by cARLA

As PD progresses, therapy becomes more complex, often requiring dose adjustments, polypharmacy, and the use of rescue treatments

Permeabilization and blocking was performed by adding PBS (Lonza) containing 0.1% saponin (Carl Roth) and 3% normal donkey serum (Abcam, Cambridge, UK) for 30 min at RT

In addition, this cream is rich in anti-aging properties, which helps to improve skin elasticity and firmness, reduce visible signs of fine lines and wrinkles, and make skin smoother and fuller

Activation of nitric oxide synthase (iNOS) or (NOS 2 ) protein in macrophage stimulates the increased secretion of nitric oxide (NO) and NO-metabolite levels within the cell