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The Strep -tag system for one-step purification and high-affinity detection or capturing of proteins

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Competition assays were done with 50 nM [ 3 H] Nisoxetine (in 1:5 molar ratio) with the concentration range for tramadol (Sigma Aldrich) being 100 nM to 3 mM, duloxetine (Sigma Aldrich) from 0.1 nM to 30 M and milnacipran (Sigma Aldrich) from 1 nM to 100 M