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Given the ancient origin of the Ca 2+ -based signaling, which evolutionary developed earlier than the mitochondrial apoptotic toolkit whose emergence cannot be dated back to the times earlier than eukaryogenesis and acquisition of an endosymbiotic mitochondrion, it can be assumed that the Ca 2+ toolkit takes over a key role in cell death signaling in terminally differentiated mitochondria-free erythrocytes

Ethical approval This study received ethical approval from the Ethics Committee of the Eye, Ear, Nose, and Throat (Eye & ENT) Hospital of Fudan University (approval number: 81470613, date of approval: 2020-06-01)

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6.2.2 Human studies The percentage of acetaldehyde retained by 8 volunteers inhaling acetaldehyde vapour (100-800 mg/m 3 ) from a recording respirometer ranged from 45 to 70%, at different respiratory rates

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