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DOI: Publisher Name: Humana Press Print ISBN: 978-1-58829-332-9 Online ISBN: 978-1-59259-800-7 eBook Packages: Springer Protocols

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We reasoned that this would preserve the critical initial protein precipitation achieved with the commonly used 40% acetonitrile, 40% methanol, and 20% water (AMW20) 29,30,31,32,33,34,35,36,37,38 extraction approach, which, when followed with a secondary water addition, would improve the solubility of polar metabolites (Fig

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